Abstract
Alternaria spp. of ascomycete fungi are known as major plant pathogens causing 20–80 % agricultural losses in field crops, horticultural crops, plantation crops, forest plants and also post harvest storage. Conventional methods of phyto-pathogenic Alternaria spp. identification is only based on their symptomatology of diseases, morphological, physiological and biochemical studies. However, identification based on formae specials, strains, or ecotypes within a species of Alternaria has limitations for accurate diagnostics, identification and detection. The species identification of these pathogenic groups of fungi is somewhat difficult because of their special growth conditions and morphological differences. The advancement of immunological assays and serological methods has shown more sensitivity and accuracy compared with earlier isolation techniques. Enzyme analysis, metabolic profiling and gel electrophoretic mobility of these pathogen proteins found to be useful for the detection of some strains and species. There are different rapid molecular diagnostics available for the detection of pathogenic Alternaria sp. including a range of pathogenic fungi like Fusarium and Macrophomina sp. Various methods based on detection and analysis of the internal transcribed spacer (ITS)-5.8S ribosomal DNA (rDNA) region are designated to be a powerful tool for precise and rapid laboratory diagnosis. However, the developments of polymerase chain reaction (PCR) variants which employ nucleic acid-based diagnostic techniques including reverse transcription (RT)-PCR, nested-PCR, multiplex-PCR, RT-PCR, RAPD and nucleic acid hybridizations are more rapid, sensitive, specific and reliable. Various commercial kits for in situ detections are available with reproducible results in a short time. Recently, with development of DNA micro-array technology and Fourier transform infrared spectroscopy (FTIR) has revolutionized the fungal phytopathogens identification and has become possible to detect different isolates of Alternaria. Nevertheless, the genomics and proteomics studies through bioinformatics tools are also supplementing with promising results. Though, the wide ranges of diagnostics techniques are available for detection, identification and quantification of Alternaria spp., the polyphasic approaches is best and more reliable than only molecular and serological methods. The sensitivity, specificity, rapidity, cost-effectiveness and their on-site suitability determines choice of the diagnostic methods. In management practices, integrated disease management (IDM) including physical, cultural, chemical and biological methods is the best strategy for Alternaria diseases. Judicious use of resistant germplasm and multi target fungicides provides sustainable advantage for management of diseases. However, development of bioinoculents and disease forecasting module will enable better strategies for eco-friendly disease management. Diversified group of fungi that have been reported as opportunistic pathogens, it is imperative that their specific identification be made correctly by an experienced microbiologist or mycologist. The species detection specific to Alternaria and development of diagnostics using molecular tools and technique is an upcoming challenge for the researchers and sustainable plant disease management.
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References
Abass MH (2013) A PCR ITS-RFLP method for identifying fungal contamination of date palm (Phoenix dactylifera L.) tissue cultures. Afr J Biotechnol 12(32):5054–5059
Abd-el-Kareem F (2007) Potassium or sodium bicarbonate in combination with Nerol for controlling early blight disease of potato plants under laboratory, greenhouse and field conditions. Egypt J Phytopathol 35(1):73–86
Abdalla SA, Algam SAA, Ibrahim EA, Naim AME (2014) In vitro screening of Bacillus isolates for biological control of early blight disease of tomato in Shambat soil. World J Agri Res 2(2):47–50
Agarwal A, Garg GK, Devi S, Mishra DP, Singh US (1997) Ultrastructural changes in Brassica leaves caused by Alternaria brassicae and destruxin B. Int J Plant Biochem and Biotechnol 6(1):25–28
Agostini JP, Bushong PM, Timmer LW (2003) Greenhouse evaluation of products that induce host resistance for control of scab, melanose, and Alternaria brown spot of citrus. Plant Dis 87:69–74
Agnes A (2009) Distribution and characterization of sweet potato Alternaria blight isolates in Uganda. A thesis submitted to the school of graduate studies in partial fullfilment of the requirement for the award of a master of science in crop science of Makerere University, Uganda
Agnihotri A, Gupta K, Prem D, Sarkar, Mehra VS, Zargar SM (2009) Genetic enhancement in rapeseed- mustard for quality and disease resistance through in vitro techniques. In: Proceedings 16th Australian Research Assembly on Brassicas, Ballarat, 14–16 Sept, p 28
Ahmad S (2009) Management of Alternaria blotch of apple using fungicides. Indian J Plant Prot 37(1 and 2):134–138
Ahmad MS, Sinha KK (2002) Mycotoxin contamination of mustard seeds during storage. Indian Phytopathol 55(3):299–302
Akamatsu H (2004) Molecular biological studies on the pathogenicity of Alternaria alternata tomato pathotype. J Gen Plant Pathol 70:389
Akamatsu H, Taga M, Kodama M, Johnson R, Otani H, Kohmoto K (1999) Molecular karyotypes for Alternaria plant pathogens known to produce hostspecific toxins. Curr Genet 35(6):647–656
Akimitsu K, Peever TL, Timmer LW (2003) Molecular, ecological and evolutionary approaches to understanding Alternaria diseases of citrus. Mol Plant Pathol 4:435–446
Akhtar KP, Saleem MY, Asghar M, Haq MA (2004) New report of Alternaria alternata causing leaf blight of tomato in Pakistan. New Dis Rep 9:43
Amein T, Wright SAI, Wikström M, Koch E, Schmitt A, Stephan D, Jahn M, Tinivella F, Gullino ML, Forsberg G, Werner S, der Wolf JV, Groot SPC (2011) Evaluation of non-chemical seed treatment methods for control of Alternaria brassicicola on cabbage seeds. J Plant Dis Prot 118(6):214–221
Amir M, Hamid M, Javad H (2014) Rapid identification of Alternaria alternata fungi, the cause of brown spot disease by loopmediated isothermal amplification. Iran J Public Health 43(2):51
Andersen B, Dongo A, Pryor BM (2008) Secondary metabolite profiling of Alternaria dauci, A. porri, A. solani & A. tomatophila. Mycol Res 112(2):241–250
Andersen B, Nielsen KF, Pinto VF, Patracia A (2015) Characterization of Alternaria strains from Argentinean blueberry, tomato, walnut and wheat. Int J Food Microbiol 196:1–10
Anderson B, Kroger E, Roberts RG (2001) Chemical and morphological segregation of Alternaria alternata, Alternaria gaisen and Alternaria longipes. Mycol Res 105:291–299
Anderson B, Hansen ME, Smedsgaard J (2005) Automated and unbiased image analysis as tool in phenotypic classification of small spored Alternaria spp. Phytopathology 95:1021–1029
Aneja JK, Agnihotri A (2013) Alternaria blight of oilseed Brassicas: epidemiology and disease control strategies with special reference to use of biotechnological approaches for attaining host resistance. J Oilseed Brassica 4(1):1–10
Aneja JK, Agarwal A, Agnihotri A (2014) Inter and intra-specific diversity in Alternaria species infecting oilseed Brassicas in India. J Oilseed Brassica 5(2):102–117
Anonymous (2005) Crop production yearbook. GPPAA- Ministério da Agricultura do Desenvolvimento Rural e das Pescas. Lisboa, Castel-Publicações e Edições AS. Appl Environ Microbiol 64(4):1313–1318. ISSN 0099-2240
Aradhya MK, Chan HM, Parfitt DE (2001) Genetic variability in the pistachio late blight fungus, Alternaria alternata. Mycol Res 105:300–306
Aroca A, Raposo R (2007) PCR-based strategy to detect and identify species of Phaeoacremonium causing grapevine diseases. Appl Environ Microbiol 73(9):2911–2918, ISSN 0099-2240
Aroca A, Raposo R, Lunello P (2008) A biomarker for the identification of four Phaeoacremonium species using the beta-tubulin gene as the target sequence. Appl Microbiol Biotechnol 80:1131–1140
Arun Kumar GS, Kamanna BC, Benagi VI (2011) Management of chrysanthemum leaf blight caused by Alternaria alternata (fr.) Keissler under field condition. Plant Arch 11(1):553–555
Atassi MZ, Lee C (1978) The precise and entire antigenic structure of native lysozyme. Biochem J 171:429–434
Avenot H, Dongo A, Simoneau NB, Iacomivasilescu B, Hamon B, Peltiers D, Simoneau P (2005) Isolation of 12 polymorphic microsatellite loci in the phytopathogenic fungus Alternaria brassicicola. Mol Ecol Notes 5:948–950
Babler E, Asensio N, Leiminger J, Hausladen H, Bahnweg G, Zinkernagel V (2004) Investigations on the appearance and control of Alternaria solani and Alternaria alternate on potato. Mitt Biol Bundesanst Land- Forstwirtschaft 396:100–101
Babu S, Seetharaman K, Nandakumar R, Johanson I (2000) Efficacy of fungal antagonists against leaf blight of tomato caused by Alternaria solani (Ell. and Mart.) Jones and Grout. J Biol Control 14(2):79–81
Balai LP, Singh RB (2013) Integration management of Alternaria blight of pigeonpea with some fungicides and antagonists in pot condition. Bioscan 8(3):881–886
Beattie SH, Holt C, Hirst D, Williams AG (1998) Discrimination among Bacillus cereus B. mycoides and B. thuringiensis and some other species of the genus Bacillus by Fourier transform infrared spectroscopy. FEMS Microbiol Lett 164:201–206
Berkeley MJ (1836) Fungi. In: Smith JE and Hooker JW (eds). The English Flora. Vol. V, Part II. Longman, London. pp 339–340
Bhardwaj SK, Laura JS (2007) Antifungal potential of some botano-extracts against Alternaria brassicae. J Plant Dis Sci 2(2):135–137
Bock CH, Peter HT, Burbaker CL, Burdon JJ (2002) Detection of genetic variation in Alternaria brassicicola using AFLP fingerprinting. Mycol Res 106(4):428–434
Bouws H, Wattenberg A, Zorn H (2008) Fungal secretomes – nature’s toolbox for white biotechnology. Appl Microbiol Biotechnol 80:381–388
Braaksma M, Martens-Uzunova ES, Punt PJ, Schaap PJ (2010) An inventory of the Aspergillus niger secretome by combining in silico predictions with shotgun proteomics data. BMC Genomics 11:584
Branan N, Wells TA (2007) Microorganism characterization using ATR–FTIR on an ultrathin polystyrene layer. Vib Spectrosc 44(1):192–196, http://dx.doi.org/10.1016/j.vibspec.2006.10.002
Brill LM, McClary RD, Sinclair JD (1994) Analysis of two ELISA formats and antigen preparations using polyclonal antibodies to Phomopsis longicolla. Phytopathology 84:173–179
Brun S, Madrid H, Gerrits van den Ende B, Andersen B, Marinach-Patrice C, Mazier D, de Hoog GS (2013) Multilocus phylogeny and MALDI-TOF analysis of the plant pathogenic species Alternaria dauchi and relatives. Fungal Biol 117:32–40
Brunus TD, White TJ, Taylor JW (1991) Fungal molecular systematic. Ann Rev Ecol Syst 22:525–564
Burgess T, Wingfield MJ, Wingfield BW (2001) Simple sequence repeat markers distinguish among morphotypes of Sphaeropsis sapinea. Appl Environ Microbiol 67:354–362
Carberry S, Neville CM, Kavanagh KA, Doyle S (2006) Analysis of major intracellular proteins of Aspergillus fumigatus by MALDI mass spectrometry: identification and characterisation of an elongation factor 1B protein with glutathione transferase activity. Biochem Biophys Res Commun 314:1096–1104
Carzaniga R, Fiocca D, Bowyer P, O’Connell RJ (2002) Localization of melanin in conidia of Alternaria alternata using phage antibodies. Mol Plant-Microbe Interact 15:216–224
Chahal SS, Pannu PPS (1997) Detection of Tilletia indica in wheat and T. Barclayana in rice samples and its Implication for seed certification. In: Hutchins JD, Reeves JC (eds) Seed health testing: Progress towards 21st century. CAB Direct, pp 153–158
Chattopadhyay C, Agrawal R, Kumar A, Bhar LM, Meena PD, Meena RL, Khan SA, Chattopadhyay AK, Awasthi RP, Singh SN, Chakravarthy NVK, Kumar A, Singh RB, Bhunia CK (2005) Epidemiology and forecasting of Alternaria blight of oilseed Brassica in India – a case study. J Plant Dis Prot 112(4):351–365
Chen RS, Chu S, Cheng C-W, Chen W-Y, Tsay J-G (2008) Differentiation of two powdery mildews of sunflower (Helianthus annuus) by a PCR-mediated method based on ITS sequences. Eur J Plant Pathol 121:1–8
Chen M, Chang Y, Zhang J, Ding J, Sun Q (2012) A genetic linkage map of Japanese scallop Mizuhopecten yessoensis based on Amplified Fragment Length Polymorphism (AFLP) and Microsatellite (SSR) markers. Afr J Biotechnol 11(46):10517–10526
Cheng DY, Zhen Y, Zhen YY, Lian BX (2008) Inhibitory activity against plant pathogenic fungi of extracts from Myoporum bontioides. A Gray and indentification of active ingredients. Pest Manag Sci 64(2):203–207
Choi YP, Paul NC, Lee HB, Yu SH (2014) First record of Alternaria simsimi causing leaf spot on sesame (Sesamum indicum L.) in Korea. Mycobiology 42(4):405–408
Chou HH, Wu WS (2002) Phylogenetic analysis of internal transcribed spacer regions of the genus Alternaria and the significance of filament-beaked conidia. Mycol Res 106:164–169
Choulwar AB, Datar VV, Kurundkar BD (1989) Efficacy of fungitoxicants on the mycelial growth of A. solani. Pestology 13:17–19
Chowdappa P, Lakshmi MJ (2013) Identification of Alternaria species associated with leaf blights of fruit, vegetable and oil seed crops based on protein and multi-locus enzyme finger prints. Pest Manag Horticult Ecosyst 19(1):45–56
Chu PWG, Waterhouse PM, Martin RR, Gerlach WL (1989) New approaches to the detection of microbial plant pathogens. Biotechnol Genet Eng Rev 7:45–111
Chupp C, Sherf AF (1960) Crucifer diseases IK. In: Vegetable diseases and their control. The Ronald Press Company, New York, Chapter 8, pp 237–288
Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme linked immuno-sorbent assay for the detection of plant viruses. J Gen Virol 34:475–483
Collier L, Balows A, Sussman M (1998) Topley & Wilson’s microbiology and microbial infections, vol 4, 9th edn. Arnold, London
Conn KL, Tewari JP (1990) Survey of Alternaria blackspot and Sclerotinia stem rot in central Alberta in 1989. Can Plant Dis Surv 70:66–67
Conn KL, Tewari JP, Dahiya JS (1988) Resistance to Alternaria brassicae and phytoalexin elicitation in rapeseed and other crucifers. Plant Sci 56:21–25
Cooke DEL, Forster JW, Jenkins PD, Gareth DJ, Lewis DM (1998) Analysis of intraspecific and interspecific variation in the genus Alternaria by the use of RAPD-PCR. Ann Appl Biol 132:197–209
Corlett M, Corlett M (1999) Fungi Canadensis No 341 Alternaria linicola. Can J Plant Pathol 21:55–57
de Hoog GS, Horre R (2002) Molecular taxonomy of the Alternaria and Ulocladium species from humans and their identification in the routine laboratory. Mycoses 45:259–276
Denis O, Van Cauwenberge A, Treutens G, Es Saadi B, Symoens F, Popovic N, Huygen K (2012) Characterization of new Alternaria alternata specific rat monoclonal antibodies. Mycopathologia 173(2–3):151–162
Deverall BJ (1995) Plant protection using natural defense systems of plants. Adv Plant Pathol 11:211–228
Dube JP (2014) Characterization of Alternaria alternata isolates causing brown spot of potatoes in South Africa. M.Sc. thesis submitted in partial fulfillment of the requirements for the master in science (Agriculture) Plant Pathology to University of Pretoria, Pretoria, South Africa
Dubey SC, Patel B, Jha OK (2000) Chemical management of Alternaria blight of broad bean. Indian Phytopathol 53(2):213–215
Dyer PS, Fumeaux PA, Douhan G, Murray TD (2001) A multiplex PCR test for determination of mating type applied to the plant pathogens Tapesia yallundae and Tapesia acuformis. Fungal Genetic Biol 33:173–180
EFSA (2011) EFSA J. 9, 2407. http://dx.doi.org/10.2903/j.efsa.2011.2407 (97 pp, www.efsa.europa.eu/efsajournal)
El_Komy MH, Saleh AA, Molan YY (2012) Molecular characterization of early blight disease resistant and susceptible potato cultivars using random amplified polymorphic DNA (RAPD) and simple sequence repeats (SSR) markers. Afr J Biotechnol 11(1):37–45
Elliott JA (1917) Taxonomic characters of the genera Alternaria and Macrosporium. Am J Bot 4:439–476
Ellis MB (1971) Dematiaceous hyphomycetes. Commonwealth Mycological Institute, Kew, pp 464–497
Ellis MB (1976) More dematiaceous hyphomycetes. Commonwealth Mycological Institute, Kew, pp 411–427
Erukhimovitch V, Tsror L, Hazanovsky M, Talyshinsky M, Mukmanov I, Souprun Y, Huleihel M (2005) Identification of fungal phyto-pathogens by Fourier transform infrared (FTIR) microscopy. J Agric Technol 1(1):145–152
Fernandez D, Ouinten M, Tantaoui A, Geiger JP, Daboussi MJ, Langin T (1998) Fot 1 insertions in the Fusarium oxysporum f. sp. albedinis genome provide diagnostic PCR targets for detection of the date palm pathogen. Appl Environ Microbiol 64:633–636
Filajdic N, Sutton TB (1992) Influence of temperature and wetness duration on infection of apple leaves and virulence of different isolates of Alternaria mali. Phytopathology 82:1279–1283
Fischer G, Braun S, Thissen R, Dott W (2006) FT-IR spectroscopy as a tool for rapid identification and intra-species characterization of airborne filamentous fungi. J Microbiol Meth 64:63–77
Fleck SC, Burkhardt B, Pfeiffer E, Metzler M (2012) Alternaria toxins: altertoxin II is a much stronger mutagen and DNA strand breaking mycotoxin than alternariol and its methyl ether in cultured mammalian cells. Toxicol Lett 214:27–32
Foster SJ, Ashby AM, Fitt BDL (2002) Improved PCR-based assays for presymptomatic diagnosis of light leaf spot and determination of mating type of Pyrenopeziza brassicae on winter oilseed rape. Eur J Plant Pathol 108:374–383
Fraaije B, Lovell DJ, Rohel EA, Hollomon DW (1999) Rapid detection and diagnosis of Septoria tritci epidemics in wheat using a polymerase chain reaction/Pico green assay. J Appl Microbiol 86:701–708
Fraaije BA, Lovell DJ, Coelho JM, Baldwin S, Hollomon DW (2001) PCR-based assays to assess wheat varietal resistance to blotch (Septoria tritici and Stagonospora nodorum) and rust (Puccinia striiformis and Puccinia recondita) diseases. Eur J Plant Pathol 107(9):905–917, ISSN 0929-1873
Ganie SA, Ghani MY, Nissar Q, Shabir-u-Rehman (2013) Bioefficacy of plant extracts and biocontrol agents against Alternaria solani. Afr J Microbiol Res 7(34):4397–4402
Gannibal PB, Klemsdal SS, Levitin MM (2007) AFLP analysis of Russian Alternaria tenuissima populations from wheat kernels and other hosts. Eur J Plant Pathol 119:175–182
Gargouri-Kammoun L, Bensassi F, Mnari-Hattab M, Rhouma A, Bacha H, Hajlaoui MR (2014) Identification of Alternaria species recovered from stored durum wheat kernels in Tunisia. Tunis J Plant Prot 9:119–129
Geiser DM, Jiménez-Gasco M, Kang S, Makalowska I, Veeraraghavan N, Ward TJ, Zhang N, Kuldau GA, O’Donnell K (2004) Fusarium-ID v.1.0: A DNA sequence database for identifying Fusarium. Eur J Plant Pathol 110(5–6):473–479, ISSN 0929-1873
Gherbawy YAMH (2005) Genetic variation among isolates of Alternaria spp. from selected Egyptian crops. Arch Phytopathol Plant Protect 38(2):77–89
Goodwin DC, Lee SB (1993) Microwave miniprep of total genomic DNA from fungi, plants, protists and animals for PCR. Biotechniques 15:438–444
Grote D, Olmos A, Kofoet JJ, Tuset E, Bertolini E, Cambra M (2002) Specific and sensitive detection of Phytophthora nicotianae by simple and nested-PCR. Eur J Plant Pathol 108(3):197–207, ISSN 0929-1873
Guglielmo F, Bergemann SE, Gonthier P, Nicolotti G, Garbelotto M (2007) A multiplex PCR-based method for the detection and early identification of wood rotting fungi in standing trees. J Appl Microbiol 103:1490–1507, ISSN 1364-5072
Guillemette T, Iacomi-Vasilescu B, Simoneau P (2004) Conventional and real-time PCR based assay for detecting pathogenic Alternaria brassicae in cruciferous seed. Plant Dis 88:490–496
Guo LD, Hyde KD, Liew ECY (2000) Identification of endophytic fungi from Livistona chinensis (Palmae) using morphological and molecular techniques. New Phytol 147:617–630
Guo-yin T, Zhi-ling Y, Zhi-lin Y, Shou-an Z (2013) Morphological, molecular and pathogenic characterization of Alternaria longipes, the fungal pathogen causing leaf spot on Atractylodes macrocephala. Afr J Microbiol Res 7(21):2589–2595
Gupta MJ, Irudayaraj JM, Debroy C, Schmilovitch Z, Mizrach A (2005) Differentiation of food pathogens using FTIR and artificial neural networks. Trans ASAE 48:1889–1892
Gupta K, Prem D, Agnihotri A (2010) Pyramiding white rust and Alternaria resistance in low erucic Brassica juncea using B. carinata. J Oilseed Brassica 1(2):55–65
Hampton JG, Trivedi RS, Townshend JM (2012) Can crop rotation reduce inoculum of the carrot pathogen Alternaria radicina? Agron N Z 42:155–162
Harrison JG, Barker H, Lowe R, Rees EA (1990) Estimation of amounts of Phytophthora infestans mycelium in leaf tissue by enzyme-linked immunsorbent assay. Plant Pathol 39:274–277
Hatta R, Ito K, Hosaki Y, Tanaka T, Tanaka A, Yamamoto M, Akimitsu K, Tsuge T (2002) A conditionally dispensable chromosome controls host-specific pathogenicity in the fungal plant pathogen Alternaria alternata. Genetics 161(1):59–70
Hayden MJ, Good G, Sharp PJ (2002) Sequence tagged microsatellite profiling (STMP): improved isolation of DNA sequence flanking target SSRs. Nucleic Acids Res 30:e129
Helm D, Naumann D (1995) Identification of some bacterial cell components by FT-IR spectroscopy. FEMS Microbiol Lett 126:75–80
Hensen JM, French R (1993) The polymerase chain reaction and plant disease diagnosis. Ann Rev Phytopathol 31:81–109
Hirsch PR, Mauchline TH, Mendum TA, Kerry BR (2000) Detection of the nematophagous fungus Verticillium chlamydosporium in nematode-infested plant roots using PCR. Mycol Res 104:435–439
Hong SG, Maccaroni M, Figuli PJ, Pryor BM, Belisario A (2006) Polyphasic classification of Alternaria isolated from hazelnut and walnut fruit in Europe. Mycol Res 110:1290–1300
Hong SY, Kang MR, Cho EJ, Kim HK, Yun SH (2010) Specific PCR detection of four quarantine Fusarium Species in Korea. Plant Pathol J 26(4):409–416, ISSN 1598-2254
Hu J, Chen C, Peever T, Dang H, Lawrence C, Mitchell T (2012) Genomic characterization of the conditionally dispensable chromosome in Alternaria arborescens provides evidence for horizontal gene transfer. BMC Genomics 13:171
Humpherson-Jones FM (1989) Survival of Alternaria brassicae and Alternaria brassicicola on crop debris of oilseed rape and cabbage. Ann Appl Biol 115:45–50
Humpherson-Jones FM, Phelps K (1989) Climatic factors influencing spore production in Alternaria brassicae and Alternaria brassicicola. Ann Appl Biol 114:449–458
Hwang BK, Yun JH, Kim ZS (1987) Geographic variation of esterase isozymes in populations of Alternaria mali on apple leaves. Phytopathol Z 119:225–231
Hyakumachi M, Priyatmojo A, Kubota M, Fukui H (2005) New anastomosis groups, AG-T and AG-U, of binucleate Rhizoctonia causing root and stem rot of cut-flower and miniature roses. Phytopathology 95:784–792
Janisiewicz WJ, Korsten L (2002) Biological control of postharvest diseases of fruits. Ann Rev Phytopathol 40:411–441
Jasalavich CA, Morales VM, Pelcher LE, Seguin-Swartz G (1995) Comparison of nuclear ribosomal DNA sequences from Alternaria species pathogenic to crucifers. Mycol Res 99:604–614
Jerne NK (1960) Immunological speculations. Annu Rev Microbiol 14:341–358
Johnson LJ, Johnson RD, Akamatsu H, Salamiah A, Otani H, Kohmoto K, Kodama M (2001) Spontaneous loss of a conditionally dispensable chromosome from the Alternaria alternata apple pathotype leads to loss of toxin production and pathogenicity. Curr Genet 40(1):65–72
Joly P (1959) Morphological variations and the idea of species in the genus Alternaria. Bull Soc Mycol Fr 75:149–158
Joly P (1964) In: Le Genre Alternaria (Encycl. Mycol.) 33:213
Kakvan N, Zamanizadeh H, Morid B, Taheri H, Hajmansor S (2012) Study on pathogenic and genetic diversity of Alternaria alternata isolated from citrus hybrids of Iran, based on RAPD-PCR technique. European J Exp Biol 2(3):570–576
Kameniecki M, Wright ER, Rivera MC (2013) Identification of Alternaria spp. as Pathogenic on the native species Terminalia australis and Salvia guaranitica. Am J Plant Sci 4:36–41
Kaminskyj S, Jilkine K, Szeghalmi A, Gough K (2008) High spatial resolution analysis of fungal cell biochemistry – bridging the analytical gap using synchrotron FTIR spectromicroscopy. FEMS Microbiol Lett 284(1):1–8, http://dx.doi.org/10.1111/j.1574-6968.2008.01162.x
Kannan P, Pandey D, Gupta AK, Punetha H, Taj G, Kumar A (2012) Expression analysis of MAP2K9 and MAPK6 during pathogenesis of Alternaria blight in Arabidopsis thaliana ecotype Columbia. Mol Biol Rep 39:4439–4444
Kantwa SL, Tetarwal JP, Shekhawat, K (2014) In vitro effect of fungicides and phyto-extracts against Alternaria alternata causing leaf blight of groundnut. 7(6) Ver. I: 28–31
Kanrar S, Venkateswari JC, Kirti PB, Chopra VL (2002) Transgenic expression of hevein, the rubber tree lectin in Indian mustard confers protection against Alternaria brassicae. Plant Sci 162(3):441–448
Katiyar A, Kant S, Chauhan SS, Alka S (2001a) Chemical control of Alternaria leaf spot of bottle gourd. Ann Plant Prot Sci 9(2):339–341
Katiyar A, Kant S, Chauhan SS, Alka S (2001b) Sources of resistance in bottle gourd to Alternaria leaf spot. Ann Plant Prot Sci 9(1):155–157
Kellens JTC, Peumans WJ (1991) Biochemical and serological comparison of lectins from different anastomosis groups of Rhizoctonia solani. Mycol Res 95:1235–1241
Kim Y, Nandakumar MP, Marten MR (2008) The state of proteome profiling in the fungal genus Aspergillus. Brief Funct Genomic Proteomic 7:87–94
Kim YT, Cho M, Jeong JY, Lee HB, Kim SB (2010) Application of terminal restriction fragment length polymorphism (T-RFLP) analysis to monitor effect of biocontrol agents on rhizosphere microbial community of hot paper (Capsicum annum L.). J Microbiol 48(5):566–572
King SR (1994) Screening, selection, and genetics of resistance to Alternaria diseases in Brassica oleracea. Ph.D. thesis, Cornell University, Ithaca
Kirk PM, Cannon PF, Minter DW, Stalpers JA (2008) Dictionary of the fungi, 10th edn. CABI, Wallingford, p 22
Klessig DF, Malamy J (1994) The salicylic acid signal in plants. Plant Mol Biol 26:1439–1458
Klimyuk VI, Carroll BJ, Thomas CM, Jones JDG (1993) Alkali treatment for rapid preparation of plant material for reliable PCR analysis. Plant J 3:493–494
Knutsen AK, Torp M, Holst-Jensen A (2004) Phylogenetic analyses of the Fusarium poae, Fusarium sporotrichioides and Fusarium langsethiae species complex based on partial sequences of the translation elongation factor-1 alpha gene. Int J Food Microbiol 95(3):287–295, ISSN 0168-1605
Köhler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predetermined specificity. Nature (Lond) 256:495–497
Kolte SJ (1996) Diseases. In: Chopra VL, Prakash S (eds) Oilseeds and vegetable brassicas. An Indian perspective. Oxford & IBH Publishing Co. Pvt. Ltd, New Delhi, pp 184–207. 294 pages
Konstantinova P, Bonantis PJM, Gent-Pelzer MPE, Van Der Zouwen P, van Der Bulk R (2002) Development of specific primers for detection and identification of Alternaria spp. in carrot material by PCR and comparison with blotter and plating assays. Mycol Res 106:23–33
Kraft JM, Boge WL (1994) Development of an antiserum to quantify Aphanomyces euteiches in resistant pea lines. Plant Dis 78:179–183
Krishnia SK, Saharan GS, Singh D (2000) Genetics of Alternaria blight resistance in inter and intraspecific crosses of Brassica juncea and B. carinata. Ann Biol 16(2):212–216
Kristensen R, Torp M, Kosiak B, Holst-Jensen A (2005) Phylogeny and toxigenic potential is correlated in Fusarium species as revealed by partial translation elongation factor 1 alpha gene sequences. Mycol Res 109(2):173–186, ISSN 0953-7562
Kumar S, Mehta N, Sangwan MS, Kumar R (2004) Relative sensitivity of various isolates of Alternaria brassicae (Berk.) Sacc. to fungicides. J Mycol Plant Pathol 34(1):28–32
Kusaba M, Tsuge T (1995) Phylogeny of Alternaria fungi known to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA. Curr Genet 28:491–498
Lacomi-Vasilescu B, Blancard D, Guénard M, Molinero-Demilly V, Laurent E, Simoneau P (2002) Development of a PCR based diagnostic assay for detecting pathogenic Alternaria species in cruciferous seeds. Seed Sci Technol 30:87–95
Lamprell H, Mazerolles G, Kodjo A, Chamba JF, Noel Y, Beuvier E (2006) Discrimination of Staphylococcus aureus strains from different species of Staphylococcus using Fourier transform infrared (FTIR) spectroscopy. Int J Food Microbiol 108:125–129
Langrell SRH, Glen M, Alfenas AC (2008) Molecular diagnosis of Puccinia psidii (guava rust) – a quarantine threat to Australian eucalypt and Myrtaceae biodiversity. Plant Pathol 57(4):687–701, ISSN 0032-0862
Le TN, Shiesh CC, Lin HL (2010) Effect of vapor heat and hot water treatments on disease incidence and quality of Taiwan native strain mango fruits. Int J Agric Biol 12:673–678
Lee SB, Taylor JW (1990) Isolation of DNA from fungal mycelia and single spores. In: Innis MA, Gelfand DH, Sninsky JS, White TJ (eds) PCR protocols: a guide to methods and applications. Academic, London, pp 282–287
Lefier D, Hirst D, Holt C, Williams AG (1997) Effect of sampling procedure and strain variation in Listeria monocytogenes on the discrimination of species in the genus Listeria by Fourier transform infrared spectroscopy and canonical variates analysis. FEMS Microbiol Lett 147:45–50
Leiminger J, Bahnweg G, Hausladen H (2014) Differentiation of Alternaria species and quantification of disease development using real-time PCR. In Fourteenth Euroblight Workshop PPO – Special report no. 16:189–194
Lievens B, Van Baarlen P, Verreth C, Van Kerckhove S, Rep M, Thomma BPHJ (2009) Evolutionary relationships between Fusarium oxysporum f. sp lycopersici and F. oxysporum f. sp radicis-lycopersici isolates inferred from mating type, elongation factor-1 alpha and exopolygalacturonase sequences. Mycol Res 113:1181–1191, Part: 10, ISSN 0953-7562
Lind V (1990) Isolation of antigens for serological identification of Pseudocercosporella herpotrichoides (Fron) Deighton. J Plant Dis Plant Prot 97:490–501
Liu GT, Qian YZ, Zang P et al (1992) Etiological role of Alternaria alternata in human esophageal cancer. China Med J 105(5):394–400
Logrieco A, Moretti A, Solfrizzo M (2009) Alternaria toxins and plant diseases: an overview of origin, occurrence and risks. World Mycotoxin J 2(2):129–140
Lopes MC, Martins VC (2008) Fungal plant pathogens in Portugal: Alternaria dauci. Rev Iberoam Micol 25:254–256
Lopez MM, Bertolini E, Olmos A, Caruso P, Gorris MT, Llop P, Penyalver R, Cambra M (2003) Innovative tools for detection of plant pathogenic viruses and bacteria. Int Microbiol 6:233–243
Madden L, Pennypacker SP, Macnab AA (1978) FAST, a forecast system for Alternaria solani on tomato. Phytopathology 68:1354–1358
Malone JP, Muskett AE (1997) In: Sheppard JW (ed) Seed-borne fungi. Description of 77 fungus species. International Seed Testing Association, Zurich, pp 19–20
Martin FN, Tooley PW (2003) Phylogenetic relationships among Phytophthora species inferred from sequence analysis of mitochondrially encoded cytochrome oxidase I and II genes. Mycologia 95(2):269–284, ISSN 0027-5514
Martínez SP, Snowdon R, Pons-Kühnemann J (2004) Variability of Cuban and international populations of Alternaria solani from different hosts and localities: AFLP Genetic Analysis. Eur J Plant Pathol 110(4):399–409
Martínez-García LB, Armas C, Padilla FM, Miranda JD, Pugnaie FI (2011) Shrubs influence arbuscular mycorrhizal fungi communities in semiarid environment. Soil Biol Biochem 43:682–689
Masunaka A, Ohtani K, Peever TL, Timmer LW, Tsuge T, Yamamoto M et al (2005) An isolate of Alternaria alternate that is pathogenic to both tangerines and rough lemon and produces two host selective toxins, ACT and ACR toxins. Phytopathology 95:241–247
Matharu BK, Sharma JR, Manrao MR (2006) Synthesis and antifungal potential of 2-chlorobenzal derivatives. Pestic Res J 18(2):113–115
Maude RB, Humpherson-Jones FM (1980a) The effect of iprodione on the seed-borne phase of Alternaria brassicicola. Ann Appl Biol 95:321–327
Maude RB, Humpherson-Jones FM (1980b) Studies on the seed-borne phases of dark leaf spot (Alternaria brassicicola) and grey leaf spot (Alternaria brassicae) of brassicas. Ann Appl Biol 95:311–319
McCartney HA, Doughty KS, Morton G, Booth EJ, Kightey SPJ, Landon G, West G, Walker KC, Thomas JE (1999) A study of the effect of disease on seed quality parameters of oilseed rape. In: Proceedings of 10th international rapeseed congress, Canberra, 26–29 Sept
McCartney HA, Foster SJ, Fraaije BA, Ward E (2003) Molecular diagnostics for fungal plant pathogens. Pestic Manag Sci 59:129–142
McMaugh SJ, Lyon BR (2003) Real-time quantitative RT-PCR assay of gene expression in plant roots during fungal pathogenesis. Biotechniques 34(5):982–986
Meena PD, Chattopadhyay C, Singh F, Singh B, Gupta A (2002) Yield loss in Indian mustard due to white rust and effect of some cultural practices on Alternaria blight and white rust severity. Brassica 4:18–24
Meena PD, Meena RL, Chattopadhyay C, Kumar A (2004) Identification of critical stage for disease development and biocontrol of Alternaria blight of Indian mustard (Brassica juncea). J Phytopathol 152(4):204–209
Mei ZZ, Rong C, Ronge X, Lin CX, Song L, Yong GZ, Xia J, Lin W, Cheng LP (2007) Synthesis and antifungal properties of sulfonilamide derivatives of chitosan. Carbohydr Res 342(16):2390–2395
Meng J, Wang Y (2010) Rapid detection of Phytophthora nicotianae in infected tobacco tissue and soil samples based on its Ypt1 gene. J Phytopathol 158(1):1–7, ISSN 0931-1785
Micales AJ, Bonde RM (1995) In Isozymes: methods and applications, Molecular methods in plant pathology, chapter 9
Mmbaga MT, Shi A, Kim M-S (2011) Identification of Alternaria alternata as a causal agent for leaf blight in Syringa species. Plant Pathol J 27(2):120–127
Mohsan M, Intizar-ul-Hassan M, Ali L (2011) Chemotheraptic management of alternaria black spot (Alternaria alternata) in mango fruits. J Agric Res 49(4):499–506
Mondal SN, Rashid MA, Monowar SM, Abdullah AM (1989) Efficacy of fungicides in controlling Alternaria blight of radish seed crop. Thai J Agric Sci 22:191–196
Mondal KK, Chatterjee SC, Viswakarma N, Bhatacharya RC, Grover A (2003) Chitinase mediated inhibitory activity of Brassicas transgenic on growth of Alternaria brassicae. Curr Microbiol 47:171–173
Mondal KR, Bhattacharya RC, Koundal KR, Chatterjee SC (2007) Transgenic Indian mustard (Brassica juncea) expressing tomato glucanase leads to arrested growth of Alternaria brassicae. Plant Cell Rep 26:247–252. doi:10.1007/s00299-006-0241-3
Montemurro N, Visconti A (1992) Alternaria metabolites-chemical and biological data. In: Chełkowski J, Visconti A (eds) Alternaria: biology, plant diseases and metabolites. Elsevier Sciences Publisher, Amsterdam, pp 449–557
Morris PF, Connolly MS, St clair DA (2000) Genetic diversity of Alternaria alternata isolated from tomato in California assessed using RAPDs. Mycol Res 104(3):286–292
Mostert L, Groenewald JZ, Summerbell RC, Gams W, Crous PW (2006) Taxonomy and pathology of Togninia (Diaporthales) and its Phaeoacremonium anamorphs. Stud Mycol 54:1–113, ISSN 0166-0616
Mukherjee AK, Carpa M-J, Zuchmanc R, Zivc T, Horwitza BA, Gepstein S (2010) Proteomics of the response of Arabidopsis thaliana to infection with Alternaria brassicicola. J Proteomics 73:709–720
Mule G, Susca A, Stea G, Moretti A (2004) Specific detection of the toxigenic species Fusarium proliferatum and F. oxysporum from asparagus plants using primers based on calmodulin gene sequences. FEMS Microbiol Lett 230(2):235–240
Mullis K (1987) Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol 155:335–350
Nakashima T, Ueno T, Fukami H, Taga T, Masuda H, Osaki K et al (1985) Isolation and structures of AK-Toxin I and II, host-specific phytotoxic metabolites produced by Alternaria alternata Japanese pear pathotype. Agric Biol Chem 49:807–815
Narayanasamy P (2001) Plant pathogen detection and disease diagnosis, 2nd edn. Marcel Dekker, New York
Narayanasamy P (2005) Immunology in plant health and its impact on food safety. The Haworth Press, New York
Naumann D, Helm D, Labischinski H (1991) Microbiological characterizations by FT-IR spectroscopy. Nature 351:81–82
Naumann A, Navarro-Gonzalez M, Peddireddi S, Kues U, Polle A (2005) Fourier transform infrared microscopy and imaging: detection of fungi in wood. Fungal Genet Biol 42(10):829–835, http://dx.doi.org/10.1016/ j.fgb.2005.06.003
Neergard P (1945) Danish species of Alternaria and Stemphylium. Oxford University Press, London
Nega E, Ulrich R, Werner S, Jahn M (2003) Hot water treatment of vegetable seed-an alternative seed treatment method to control seed borne pathogens in organic farming. J Plant Dis Prot 110(3):220–234
Nguyen HDT, Seifert KA (2008) Description and DNA barcoding of three new species of Leohumicola from South Africa and the United States. Persoonia 21:57–69, ISSN 0031-5850
Niessen ML, Vogel RF (1998) Group specific PCR-detection of potential trichothecene- producing Fusarium-species in pure cultures and cereal samples. Syst Appl Microbiol 21:618–631
Ostry V (2008) Alternaria mycotoxins: an overview of chemical characterization, producers, toxicity, analysis and occurrence in foodstuffs. World Mycotoxin J 1:175–188
Oudemans P, Coffey MD (1991) A revised systematic of twelve papillate Phytophthora species based on isozymes analysis. Mycol Res 95:1025–1046
Oviedo MS, Sturm ME, Reynoso MM, Chulze SN, Ramirez ML (2013) Toxigenic profile and AFLP variability of Alternaria alternata and Alternaria infectoria occurring on wheat. Braz J Microbiol 44(2):447–455
Pandey A (2010) Antagonism of two Trichoderma Species against Alternaria alternata on Capsicum frutescens. J Exp Sci 1(5):18–19
Pane C, Zaccardelli M (2015) Evaluation of Bacillus strains isolated from solanaceous phylloplane for biocontrol of Alternaria early blight of tomato. Biol Control 84:11–18
Panwar S, Singh L, Sunaina V, Vats P (2006) Evaluation of Brevibacillus brevis and Bacillus firmus strains in management of fungal pathogens. Pestic Res J 18(2):159–161
Pastor FJ, Guarro J (2008) Alternaria infections: laboratory diagnosis and relevant clinical features. Clin Microbiol Infect 14:734–746
Patni CS, Kolte SJ (2006) Effect of some botanicals in management of Alternaria blight of rapeseed-mustard. Ann Plant Prot Sci 14:151–156
Peever TL, Ibanez A, Akimitsu K, Timmer LW (2002) Worldwide phylogeography of the citrus brown spot pathogen, Alternaria alternata. Phytopathology 92:794–802
Peever TL, Su G, Carpenter-Boggs L, Timmer LW (2004) Molecular systematics of citrus-associated Alternaria species. Mycologia 96:119–134
Peres NA, Timmer LW (2006) Evaluation of the Alter-Rater model for spray timing for control of Alternaria brown spot on murcott tangor in Brazil. Crop Protec 25:454–460
Petrunak DM, Christ BJ (1992) Isozyme variability in Alternaria solani and A.alternata. Phytopathology 82:1343–1347
Porter-Jordan K, Rosenberg EI, Keiser JF, Gross JD, Ross AM, Nasim S, Garrett CT (1990) Nested polymerase chain reaction assay for the detection of cytomegalovirus overcomes false positives caused by contamination with fragmented DNA. J Med Virol 30(4):85–91, ISSN 0146- 6615
Prelle A, Spadaro D, Garibaldi A, Gullino ML (2013) A new method for detection of five Alternaria toxins in food matrices based on LC–APCI-MS. Food Chem 140:161–167
Primard C, Vedel F, Mathieu C, Pelletier G, Chevre AM (1988) Interspecific somatic hybridization between Brassica napus and Brassica hirta (Sinapis alba L.). Theor Appl Genet 75:546–552
Pryor BM, Bigelow DM (2003) Molecular characterization of Embellisia and Nimbya species and their relationship to Alternaria, Ulocladium, and Stemphylium. Mycologia 95:1141–1154
Pryor BM, Gilbertson RL (2000) Phylogenetic relationships among Alternaria and related fungi based upon analysis of nuclear internal transcribed sequences and mitochondrial small subunit ribosomal DNA sequences. Mycol Res 104:1312–1321
Pryor BM, Michailides TJ (2002) Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with Alternaria late blight of pistachio. Phytopathology 92:406–416
Pusz W (2009) Morpho-physiological and molecular analyses of Alternaria alternata isolated from seeds of Amaranthus. Phytopathologia 54:5–14
Qin L, Fu Y, Xie J, Cheng J, Jiang D, Li G, Huang J (2011) A nested-PCR method for rapid detection of Sclerotinia sclerotiorum on petals of oilseed rape (Brassica napus). Plant Pathol 60(2):271–277, ISSN 0032-0862
Radisek S, Jakse J, Javornik B (2004) Development of pathotype-specific SCAR markers for detection of Verticillium albo-atrum isolates from Hop. Plant Dis 88(10):1115–1122. doi:10.1094/PDIS.2004.88.10.1115
Ramdaswaghunde R, Patil RK, Shinde MS (2014) Occurrence and fungicidal management of Alternaria fruit rot (Alternaria altarnata (Fr.) Keissler) of Aonla. J Mycopathol Res 52(2):311–316
Ramjegathesh R, Ebenezar EG, Muthusamy M (2011) Management of onion leaf blight by Alternaria alternata (FR.) Keissler by botanicals and bio-control agents. Plant Pathol J 10(4):192–196
Ratnam SV, Reddy PN, Rao KC, Raju SK, Kumar JH (2001) Effect of salicylic acid and bion seed treatment in induction of systemic resistance against sunflower leaf blight. Alternaria helianthi (Hansf.). Tub Et Nish Indian J Plant Prot 29:79–81
Rimmer SR, Buchwaldt H (1995) Diseases. In: Kimber D, McGregor DI (eds) Brassica oilseeds-production and utilization. CAB International, Allingford, pp 111–140
Rivillas-Acevedo LA, Soriano Garcia M (2007) Isolation and biochemical characterization of an antifungal peptide from Amaranthus hypochondriacus seeds. J Agric Food Chem 55(25):10156–10161
Roberts RG (2005) Alternaria yaliinficiens sp. nov. on Ya Li pear fruit: from interception to identification. Plant Dis 89:134–145
Roberts RG, Reymond ST, Andersen B (2000) RAPD fragment pattern analysis and morphological segregation of small-spored Alternaria species and species groups. Mycol Res 104:151–160
Rotem J (1994) The genus Alternaria: biology, epidemiology and pathogenicity. American Phytopathological Society Press, St Paul
Saccardo PA (1886) Sylloge fungorum omninum hucusque cognitorum. Pavia, 4: pp. 1–807
Saito K, Takeda K (1984) Genetic analysis of resistance to Alternaria blotch (Alternariamali Roberts) in apple. (Studies on the breeding of the apple. VIII). Jpn J Breed 34:197–209
Salamiah H, Yukitaka F, Hiroshi O, Motoichiro K (2001) Construction and genetic analysis of hybrid strains between apple and tomato pathotypes of Alternaria alternata by protoplast fusion. J Gen Plant Pathol 67(2):97–105
Salman A, Erukhimovitch V, Talyshinsky M, Huleihil M (2002) FTIR spectroscopic method for detection of cells infected with herpes viruses. Biopolymers- Biospectroscopy Sect 67(6):406–412, http://dx.doi.org/10.1002/bip.10171
Samson RA, Houbraken J, Thrane U, Frisvad JC, Andersen B (2010) Food and indoor fungi. CBS-KNAW— Fungal Biodiversity Centre, Utrecht, pp 1–398
Santos C, Fraga ME, Kozakiewicz Z, Lima N (2010) Fourier transform infrared as a powerful technique for the identification and characterization of filamentous fungi and yeasts. Res Microbiol 161(2):168–175
Sawamura K (1990) Alternaria blotch. In: Aldwinckle HS (ed) Compendium of apple and pear diseases. APS Press, St Paul, p 100
Schena M, Shalon D, Davis RW, Brown PO (1995) Quantitative monitoring of gene-expression patterns with a complementary-DNA microarray. Science 270:467–470
Schenk PM, Kazan K, Manners JM, Anderson JP, Simpson RS, Wilson IW, Somerville SC, Maclean DJ (2003) Systemic gene expression in Arabidopsis during an incompatible interaction with Alternaria brassicicola. Plant Physiol 132:999–1010
Schwarz C, Tiessen C, Kreutzer M, Stark T, Hofmann T, Marko D (2012) Characterization of a genotoxic impact compound in Alternaria alternata infested rice as Altertoxin II. Arch Toxicol 86:1911–1925
Scott PM (2004) Other mycotoxins. In: Magan N, Olsen M (eds) Mycotoxins in food: detection and control. Woodhead Publishing, Cambridge, UK, pp 406–440
Seidle E, Rude S, Petrie A (1995) The effect of Alternaria black spot of canola on seed quality and seed yield and studies on disease control. Agriculture and Agri-Food Canada Saskatoon, Saskatoon, 41 pp
Seifert KA, Samson RA, deWaard JR, Houbraken J, Lévesque CA, Moncalvo J-M, Louis-Seize G, Hebert PDN (2007) Prospects for fungus identification using CO1 DNA barcodes, with Penicillium as a test case. Proc Natl Acad Sci USA 104(10):3901–3906, ISSN 0027-8424
Shakir AS, Mirza JH, Akhtar KP (1997) New records of Alternaria species from Pakistan. Pak J Phytopathol 9:102–104
Sharma TR, Singh BM (1995) Generation and evaluation of somaclones of Brassica juncea for resistance to Albugo candida and Alternaria brassicae. Proc Indian Natl Sci Acad B61(2):155–162
Sharma A, Dass A, Pau MS (2007) Antifungal effect of neem extract on some common phytopathogenic fungi. Adv Plant Sci 20(2):357–358
Sharma P, Deep S, Sharma M, Bhati DS, Chowdappa P (2013) PCR based assay for the detection of Alternaria brassicicola in Crucifers. Indian Phytopathol 66(3):263–268
Sheridan GEC, Masters CI, Shallcross JA, Mackey BM (1998) Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells. Appl Environ Microbiol 64(4):1313–1318, ISSN 0099-2240
Shin YU, Ko KC (1992) Studies on Alternaria leaf spot (A. mali Roberts) in apple trees (Malus domestica Borkh): epiphytotics, screening methods and inheritance of resistance. III. Inheritance of resistance. J Korean Soc Hortic Sci 33:372–378
Shoaib A, Akhtar N, Nafisa, Aftab A (2013) Fourier transform-infrared spectroscopy to monitor modifications in canola biochemistry caused by Alternaria destruens. Pak J Phytopathol 25(02):105–109
Shrama R, Tewari JP (1998) RAPD analysis of three Alternaria species pathogenic to crucifers. Mycol Res 102(7):807–814
Shrestha SK, Munk L, Mathur SB (2003) Survival of Alternaria brassicae in seeds and crop debris of rapeseed and mustard in Nepal. Seed Sci Technol 31(1):103–109
Siddiqui ZA (2007) Biocontrol of Alternaria triticina by plant growth promoting rhizobacteria on wheat. Arch Phytopathol Plant Protect 40(4):301–308
Sigareva MA, Earle ED (1999a) Production and characterization of somatic hybrids between Camilina sativa and rapid-cycling B. oleracea. Theor Appl Genet 98:164–170
Sigareva MA, Earle ED (1999b) Camalexin induction in intertribal somatic hybrids between Camelina sativa and rapid cycling Brassica oleracea. Theor Appl Genet 98:164–170
Sigareva MA, Earle ED (1999c) Regeneration of plants from protoplasts of Capsella bursapastoris and somatic hybridization with rapid cycling Brassica oleracea. Plant Cell Rep 18:412–417
Simmons EG (2007) Alternaria: an identification manual. Centraalbureau voor\Schimmelcultures, Utrecht
Singh N, Verma OP (2009) Epidemiology of Alternaria blight of Adhatoda vasica caused by Alternaria alternata. Indian J Agric Sci 79(11):945–948
Singh NK, Saxena RP, Pathak SP, Chauhan SKS (2001) Management of Alternaria leaf spot disease of tomato. Indian Phytopathol 54:508
Sissons JGP, Oldstone MBA (1980) Antibody-mediated destruction of virus-infected cells. Adv Immunol 29:209–260
Skrede I, Carlsen T, Stensrud O, Kauserud H (2012) Genome wide AFLP markers support cryptic species in Conphora (Boletales). Fungal Biol 116:778–784
Song W, Ma X, Tan H, Zhou J (2011) Abscisic acid enhances resistance to Alternaria solani in tomato seedlings. Plant Physiol Biochem 49:693–700
Spadaro D, Gullino ML (2004) State of the art and future prospects of the biological control of postharvest fruit diseases. Int J Food Microbiol 91:185–194
Strandberg JO (2002) A selective medium for the detection of Alternaria dauci and Alternaria radicina. Phytoparasitica 30:269–284
Strange RN (2007) Phytotoxins produced by microbial plant pathogens. Nat Prod Rep 24:127–144
Surekha C, Neelapu N, Prasad BS, Ganesh PS (2014) Induction of defense enzymes and phenolic content by Trichoderma Viride in Vigna mungo infested with Fusarium oxysporum and Alternaria alternata. Int J Agric Sci Res 4(4):31–40
Szeghalmi A, Kaminskyj S, Gough KM (2007) A synchrotron FTIR microspectroscopy investigation of fungal hyphae grown under optimal and stressed conditions (conference paper). Anal Bioanal Chem 387(5):1779–1789, http://dx.doi.org/10.1007/s00216-006-0850-2
Taj G, Kumar A, Bansal KC, Garg GK (2004) Introgression of osmotin gene for creation of resistance against Alternaria blight by perturbation of cell cycle machinery. Indian J Biotechnol 3:291–298
Takenaka S (1992) Use of immunological methods with antiribosome serums to detect snow mold fungi in wheat plants. Phytopathology 82:896–901
Taylor MJ, Hughes MS, Skuce RA, Neill SD (2001) Detection of Mycobacterium bovis in bovine clinical specimens using real-time fluorescence and fluorescence energy transfer probe rapid cycle PCR. J Clinic Microbiol 39:1272–1278
Thakur S, Harsh NSK (2014) Phylloplane fungi as biocontrol agent against Alternaria leaf spot disease of (Akarkara) Spilanthes oleracea. Biosci Discov 5(2):139–144
Thies JE (2007) Soil Microbial community analysis using terminal restriction fragment length polymorphisms. Soil Sci Soc Am J 71:579–591
Tohamy MRA, Helal GA, Ibrahim KI, Abd-El-Aziz SA (2004) Control of postharvest tomato fruit rots: II. Using heat treatments. Egypt J Phytopathol 32(1–2):129–138
Toriyama K, Hinata K, Kameya T (1987) Production of somatic hybrid plants “Brassicomoricandia”, through protoplast fusion between Moricandida arvensis and Brassica oleracea. Plant Sci 48(2):123–128
Trivedi RKS (2010) Strategies to control Alternaria radicina in carrot seed production. A Ph.D thesis submitted to Lincoln University, Seed Research Centre, Bio-Protection Research Centre, Lincoln University Canterbury, Lincoln
Tsuge T, Harimoto Y, Akimitsu K, Ohtani K, Kodama M, Akagi Y, Egusa M, Yamamoto M, Otani H (2013) Host-selective toxins produced by the plant pathogenic fungus Alternaria alternata. FEMS Microbiol Rev 37:44–66
Udelhoven T, Naumann D, Schmitt J (2000) Development of a hierarchical classification system with artificial neural networks and FT-IR spectra for the identification of bacteria. Appl Spectrosc 54:1471–1479
Upadhyay P, Rai A, Kumar R, Singh M, Sinha B (2014) Differential expression of pathogenesis related protein genes in tomato during inoculation with A. Solani. J Plant Pathol Microbiol 5:217. doi:10.4172/2157-7471.1000217
Van der Waals JE, Denner FDN, van Rij N, Korsten L (2003) Evaluation of PLANT- Plus, a decision support system for control of early blight on potatoes in South Africa. Crop Protec 22:821–828
Valkonen JPT, Koponen H (1990) The seed-borne fungi of Chinese cabbage (Brassica pekinensis), their pathogenicity and control. Plant Pathol 39:510–516
Velichetti RK, Lamison C, Brill LM, Sinclair JB (1993) Immunodetection of Phomopsis species in asymptomatic plants. Plant Dis 77:70–73
Venkataramanamma K, Madhusudhan P, Neelima S, Narasimhudu Y (2014) Field evaluation of fungicides for the management of Alternaria leaf blight of sunflower. Indian J Plant Prot 42(2):165–168
Verma PR, Saharan GS (1994) Monograph on Alternaria diseases of crucifers. Agriculture and agri-food Canada, pp 1–146
Verma N, Verma S (2010) Alternaria diseases of vegetable crops and new approaches for its control. Asian J Exp Biol Sci 1(3):681–692
Verma SS, Yajima WR, Rahman MH, Shah S, Liu JJ, Ekramoddoullah AK, Kav NN (2012) A cysteine-rich antimicrobial peptide from Pinus monticola (PmAMP1) confers resistance to multiple fungal pathogens in canola (Brassica napus). Plant Mol Biol 79(1–2):61–74
Vishwanath, Kolte SJ, Singh MP, Awasthi RP (1999) Induction of resistance in mustard (Brassica juncea) against Alternaria black spot with an avirulent Alternaria brassicae isolate-D. Eur J Plant Pathol 105(2):217–220
Waghe KP, Wagh SS, Kuldhar DP, Pawar DV (2015) Evaluation of different fungicides, bioagents and botanicals against Alternaria blight caused by Alternaria helianthi (Hansf) of sunflower. Afr J Agric Res 10(5):351–358
Ward CM, Wilkinson AP, Bramham S, Lee HA, Chan WHS, Butcher GW, Hutchings A, Morgan MRA (1990) Production and characterization of polyclonal and monoclonal antibodies against aflatoxin B1 oxime-BSA in an enzyme-linked immunosorbent assay. Mycotoxin Res 6:73–83
Welsh J, McClelland M (1991) Genomic fingerprinting using arbitrarily primed PCR and a matrix of pairwise combinations of primers. Nucleic Acids Res 19(19):5275–5279
Westman AL, Kresovich S, Dickson MH (1999) Regional variation in Brassica nigra and other weedy crucifers for disease reaction to Alternaria brassicicola and Xanthomonas campestris pv. campestris. Euphytica 106:253–259
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal genes for phylogenetics. In: PCR protocols: a guide to methods and applications. Academic, San Diego
Wijekoon CP, Goodwin PH, Hsiang T (2008) Quantifying fungal infection of plant leaves by digital image analysis using Scion Image software. J Microbiol Method 74:94–101
Wiltshire SP (1933) The foundation species of Alternaria and Microsporium. Trans Br Mycol Soc 18:135–160
Wiltshire SP (1938) The original and modern conceptions of Stemphylium. Trans Br Mycol Soc 21:211–239
Wu WS, Chen TW (1999) Development of a new selective medium for detecting Alternaria brassicola in cruciferous seeds. Seed Sci Technol 27:397–409
Wu CP, Chen GY, Li B, Su H, An YL, Zhen SZ, Ye JR (2011) Rapid and accurate detection of Ceratocystis fagacearum from stained wood and soil by nested and realtime PCR. Forest Pathol 41(1):15–21, ISSN 1437-478
Yamagishi D, Akamatsu H, Otani H, Kodama M (2006) Pathological evaluation of host-specific AAL-toxins and fumonisin mycotoxins produced by Alternaria and Fusarium species. J Gen Plant Pathol 72(5):323–327
Yang F, Jensen JD, Svensson B, Jorgensen HJL, Collinge DB, Finnie C (2010) Analysis of early events in the interaction between Fusarium graminearum and the susceptible barley (Hordeum vulgare) cultivar Scarlett. Proteomics 10(21):3748–3755, ISSN 1615-9853
Zghair FS, Mohamed BT, Neda SM (2014) Molecular assay of Polyketide Synthase gene of Alternariol (AOH) produce by Alternaria alternata. Int J Innov Appl Stud 9(3):1124–1127
Zhao Y, Tu K, Shao XF, Jing W, Yang W, Su ZP (2008) Biological control of the post-harvest pathogens Alternaria solani, Rhizopus stolonifer and Botrytis cinerea on tomato fruit by Pichia guilliermondii. J Hortic Sci Biotechnol 83(1):132–136
Zheng FC, Ward E (1998) Variation within and between Phytophthora species from rubber and citrus trees in China, determined by polymerase chain reaction using RAPDs. J Phytopathol 146(2-3):103–109
Zore GB, Surwase BS, Karuppayil SM (2004) Antifungal activity in two medicinal plants. J Mycol Plant Pathol 34(2):543–547
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Nagrale, D.T., Sharma, L., Kumar, S., Gawande, S.P. (2016). Recent Diagnostics and Detection Tools: Implications for Plant Pathogenic Alternaria and Their Disease Management. In: Kumar, P., Gupta, V., Tiwari, A., Kamle, M. (eds) Current Trends in Plant Disease Diagnostics and Management Practices. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-27312-9_5
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DOI: https://doi.org/10.1007/978-3-319-27312-9_5
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